Background :female dancers are at risk of negative energy balance and its adverse effects. However, there is no evidence regarding the energy balance of Iranian female dancers in the literature. Methods :In a cross-sectional study of 25 female nonprofessional dancers, dietary intake was assessed using self-reported food diaries and recall interviews on three different days: One dance day, one workday without dance, and one weekend day without dance. Nutrient intake was analyzed and converted into protein, carbohydrate, and fat components, and subsequently into energy equivalents. Total energy expenditure was calculated based on physical activity measurements taken on dance, work, and rest days. The average daily energy intake and expenditure over a 7-day period were then compared. Results :Significant daily energy deficiency was observed based on the average over 7 days (- 772.7 kcal +/- 450 kcal, P-value < 0.05). Dancers consumed less protein and carbohydrates and more fat than the recommended daily values (P-value < 0.05). Conclusions :It appears that Iranian female dancers suffer from negative energy balance and nutritional malpractice. This may be related to underlying eating disorders or perceptions of body image, as well as inadequate nutritional knowledge. Consulting experienced sports nutritionists and other necessary specialists is recommended.